Journal: bioRxiv

Article Title: Building an atlas of mechanobiology: high-throughput contractility screen of 2418 kinase inhibitors in five primary human cell types reveals selective divergent responses among related cell types

doi: 10.1101/2025.01.11.632556

Figure Lengend Snippet: C o mparing responses in HLF-SC and HHSteC shows both general and selective responses. (a) Scatter plot of average z-scores for contraction for compounds tested in both cell types at the confirmation screen level. Note: not all hits were tested in both cell types, as in this case molecules were only re-tested in the cell type where they were originally identified as hits. (b) Distribution of compounds by affected pathways for confirmed hits in each cell type, highlighting pathway overrepresentation. (c) Dose response curves for selected compounds demonstrating cell-type selectivity among the MYO cells. Compounds labelled with an asterisk (*) were over 10 times more potent (by IC50) in HHSteC.

Article Snippet: Cryopreserved human primary lung fibroblasts (HLF) and human tracheal), human primary hepatic stellate cells (HHSteC), human airway smooth muscle (HASM) cells, and human bladder smooth muscle (HBSM) cells were obtained from ScienCell.

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